Changes in ependymal zone proliferation following contusion SCI.

<p>(<b>A</b>) Immunohistochemistry for Ki67-positive (+) proliferating cells in each harvested segment of the spinal cord of naive mice and 3, 14, or 35 days post-injury (dpi). Sections are counterstained with cresyl violet. Note the increased cell proliferation in the ependymal zone of the Medial, Rostral and Cervical spinal cord segments in SCI mice compared to naive animals. (<b>B</b>) Multilabel fluorescence immunostaining of the ependymal zone at 7 dpi. Upper panel shows immunostaining for Vimentin (orange) and Ki67 (green) while lower panel shows the same field labeled with Olig2 (fucia) and Hoechst counterstaining (blue). Note that Vimentin+/Ki67+ proliferating EpCs can be seen leaving the central canal ependymal zone (arrowheads). (<b>C</b>) Graph of the spatial and temporal progression of changes in ependymal zone proliferation following contusion SCI. Scale bars: <b>A</b>, 40 µm; <b>B</b>, 35 µm.