Sorting living cervical cancer cells using the nuclear protein marker Sox2.

<p>Sox2 protein expression was detected by western blot (A) and immunohistochemistry (B) in the cervical cancer cell lines HeLa, SiHa, CaSki, and C33A. (C) The abundance of EGFP-positive tumor cells in cervical cancer cell lines transfected with the pSox2/EGFP reporter. (D) Immunohistochemical analysis of EGFP⁺ and EGFP⁻ cells. (E) Sox2 protein expression in a monolayer, tumorsphere cells, and sorted EGFP⁺ and EGFP⁻ cells.