<p>(A) CD4<sup>+</sup> T cells were polarized toward Th1 cells for 4 days in conditioned medium from unstimulated or LPS-stimulated astrocytes supplemented with 10 ng/mL IL-12 (Th1) or 5 ng/mL TGFβ (Treg). Cells were washed and stimulated for 4 h with PMA/ionomycin/BFA and IFNγ-producing CD4<sup>+</sup> T and Foxp3 expressing CD4<sup>+</sup> T cells were analyzed by flow cytometry. Flow cytometry analyses were quantified and expressed as mean ± s.e.m. (n = 2). (B–C) Different quantities of CD4<sup>+</sup> T cells were cultivated on an astrocyte monolayer and polarized toward (B) Th1, or (C) Treg cells for 4 days in medium supplemented with 10 ng/mL IL-12 (Th1), or 5 ng/mL TGFβ (Treg). Cells were washed, stimulated for 4 h with PMA/ionomycin...
<p>(A) Representative images of IL-4 or IFN-γ positive CD4<sup>+</sup> T cells after co-culturation ...
SummaryFollicular helper T (Tfh) cells comprise an important subset of helper T cells; however, thei...
<p>Splenocytes were harvested from C57B6 mice and bulk CD4<sup>+</sup> T cells were isolated. CD4<su...
<p>CD4<sup>+</sup> T cells were cultivated on astrocytes in the presence of 10 µg/mL anti-IL-4 alone...
<p>(A) CD4<sup>+</sup> T cells were cultivated for 4 days on no feeder cells or on a monolayer of as...
<p>(A) CD4<sup>+</sup> T cells were polarized toward Th1 cells for 3 days cultured on astrocytes or ...
<p>CD4<sup>+</sup> T cells were cultivated for 4 days on a monolayer of astrocytes that were pre-act...
T-cell characteristics are dynamic and influenced by multiple factors. To test whether cells and the...
T-cell characteristics are dynamic and influenced by multiple factors. To test whether cells and the...
Astrocytes usually respond to trauma, stroke, or neurodegeneration by undergoing cellular hypertroph...
<p>(A) CD4<sup>+</sup> T cells were cultivated on irradiated splenocytes in the presence of 10 µg/mL...
STAT4 is an essential transcription factor for Th1 cell development. IL-12 and IFN-α both activate S...
<p>(A–E) FACS-sorted naive CD4<sup>+</sup>CD62L<sup>hi</sup>CD44<sup>lo</sup>CD25<sup>−</sup>CXCR3<s...
Using a human culture system, we have previously shown that interferon-y-and tumour necrosis factor-...
International audienceWhen helper T (TH) cell polarization was initially described three decades ago...
<p>(A) Representative images of IL-4 or IFN-γ positive CD4<sup>+</sup> T cells after co-culturation ...
SummaryFollicular helper T (Tfh) cells comprise an important subset of helper T cells; however, thei...
<p>Splenocytes were harvested from C57B6 mice and bulk CD4<sup>+</sup> T cells were isolated. CD4<su...
<p>CD4<sup>+</sup> T cells were cultivated on astrocytes in the presence of 10 µg/mL anti-IL-4 alone...
<p>(A) CD4<sup>+</sup> T cells were cultivated for 4 days on no feeder cells or on a monolayer of as...
<p>(A) CD4<sup>+</sup> T cells were polarized toward Th1 cells for 3 days cultured on astrocytes or ...
<p>CD4<sup>+</sup> T cells were cultivated for 4 days on a monolayer of astrocytes that were pre-act...
T-cell characteristics are dynamic and influenced by multiple factors. To test whether cells and the...
T-cell characteristics are dynamic and influenced by multiple factors. To test whether cells and the...
Astrocytes usually respond to trauma, stroke, or neurodegeneration by undergoing cellular hypertroph...
<p>(A) CD4<sup>+</sup> T cells were cultivated on irradiated splenocytes in the presence of 10 µg/mL...
STAT4 is an essential transcription factor for Th1 cell development. IL-12 and IFN-α both activate S...
<p>(A–E) FACS-sorted naive CD4<sup>+</sup>CD62L<sup>hi</sup>CD44<sup>lo</sup>CD25<sup>−</sup>CXCR3<s...
Using a human culture system, we have previously shown that interferon-y-and tumour necrosis factor-...
International audienceWhen helper T (TH) cell polarization was initially described three decades ago...
<p>(A) Representative images of IL-4 or IFN-γ positive CD4<sup>+</sup> T cells after co-culturation ...
SummaryFollicular helper T (Tfh) cells comprise an important subset of helper T cells; however, thei...
<p>Splenocytes were harvested from C57B6 mice and bulk CD4<sup>+</sup> T cells were isolated. CD4<su...