Abnormal proliferation and differentiation at the growth plate in <i>Fgfr2^+/S252W</i> mice from P10.

<p>(A–H) Col 2, Col 10, Cbfa1 and OC immunoreactivity in the tibia epiphyses. Immunolocalization (brown color) of both chondrocyte and osteocyte markers in the entire epiphyseal area were reduced in MT mice at P10 (WT: A–D, MT: E–H). (I, J) PCNA immunohistochemistry revealed a significantly reduced number of proliferating chondrocytes in the tibia growth plate of P10 MT mice. (K) The percentage of PCNA-labeled cells was reduced in the growth plates of the MT mice. (L, M) TUNEL assay revealed no difference in apoptosis in the tibia growth plate of the two types at P5. (N) The Percentage of TUNEL+ labeled cells was similar in the two groups. Data are presented as the mean ±SD. **<i>P</i><0.01. Scale bars = 100 µm.