<p>(A) Alcian blue staining showed reduced numbers of blue-staining cells in cultured BMSCs from MT mice compared with WT on days 14 and 21. (B) Alizarin red staining of the mineralized osteoblasts showed reduced numbers of mineralized nodules on days 14 and 21 in MT mice. (C) MTT proliferation assays showed reduced proliferation of BMSCs from MT mice. (D) Histochemical staining shows that Col 2 expression was lower in MT mice than in WT after chondrogenic induction. (E) Relative expression of early chondrogenic differentiation genes measured by RT-PCR. The expression levels of <i>Col2</i> and <i>Col10</i> mRNA in differentiated BMSCs were markedly reduced in BMSCs from MT mice at 2 and 3 weeks. (F) Relative expression of late chondrogenic ...
In this study, we investigated the influence of transforming growth factor beta 3 (TGF-beta3), bone ...
International audienceAdult mesenchymal stem cells (MSCs) are currently being investigated as an alt...
<p>(A) CCK-8 assay showed that <i>Fgfr2</i><sup>S252W/+</sup> BMSCs proliferated more slowly than wi...
<p>(A) ALP staining showed less crystal violet-staining cells in cultured <i>Fgfr2</i><sup>S252W/+</...
SummaryObjectiveTo test the effects of sequential exposure to FGF2, 9 and 18 on human Mesenchymal St...
<p>(A–H) Col 2, Col 10, Cbfa1 and OC immunoreactivity in the tibia epiphyses. Immunolocalization (br...
<p>(A) Western blotting demonstrated that levels of phospho-Erk1/2 and phospho-p38 were both increas...
We previously generated separate lines of transgenic mice that specifically overexpress either the F...
<p>(A, B) Pre-exposure to FGF2 prior to differentiation stimuli inhibits osteoblast differentiation ...
<p>Sections of the tibia from 8-week-old mice injected with tamoxifen for 4 weeks were analyzed by h...
Adult mesenchymal stem cells (MSCs) are considered promising candidate cells for therapeutic cartila...
<p>(A) MTT assay. Primary osteoblasts from calvariae of 4 wild-type and 3 Bcl2<sup>−/−</sup> mice we...
<p>Total RNA was extracted from ATDC5 cultured in ITS conditions for 14 or 28 days and stimulated or...
The differentiation of multipotential mesenchymal cells into chondrocytes and osteoblasts is a funda...
<p>(2A): The histological section of mandibular condylar cartilage from a 4 week-old-mice (Dkk3xCol2...
In this study, we investigated the influence of transforming growth factor beta 3 (TGF-beta3), bone ...
International audienceAdult mesenchymal stem cells (MSCs) are currently being investigated as an alt...
<p>(A) CCK-8 assay showed that <i>Fgfr2</i><sup>S252W/+</sup> BMSCs proliferated more slowly than wi...
<p>(A) ALP staining showed less crystal violet-staining cells in cultured <i>Fgfr2</i><sup>S252W/+</...
SummaryObjectiveTo test the effects of sequential exposure to FGF2, 9 and 18 on human Mesenchymal St...
<p>(A–H) Col 2, Col 10, Cbfa1 and OC immunoreactivity in the tibia epiphyses. Immunolocalization (br...
<p>(A) Western blotting demonstrated that levels of phospho-Erk1/2 and phospho-p38 were both increas...
We previously generated separate lines of transgenic mice that specifically overexpress either the F...
<p>(A, B) Pre-exposure to FGF2 prior to differentiation stimuli inhibits osteoblast differentiation ...
<p>Sections of the tibia from 8-week-old mice injected with tamoxifen for 4 weeks were analyzed by h...
Adult mesenchymal stem cells (MSCs) are considered promising candidate cells for therapeutic cartila...
<p>(A) MTT assay. Primary osteoblasts from calvariae of 4 wild-type and 3 Bcl2<sup>−/−</sup> mice we...
<p>Total RNA was extracted from ATDC5 cultured in ITS conditions for 14 or 28 days and stimulated or...
The differentiation of multipotential mesenchymal cells into chondrocytes and osteoblasts is a funda...
<p>(2A): The histological section of mandibular condylar cartilage from a 4 week-old-mice (Dkk3xCol2...
In this study, we investigated the influence of transforming growth factor beta 3 (TGF-beta3), bone ...
International audienceAdult mesenchymal stem cells (MSCs) are currently being investigated as an alt...
<p>(A) CCK-8 assay showed that <i>Fgfr2</i><sup>S252W/+</sup> BMSCs proliferated more slowly than wi...