Cell targeting and spike train auto-correlograms.

<p><b>A.</b> Fluorescence images of retina in presence of bath applied sulforhodamine, a counterstain showing intact cell bodies (dark) on lighter fluorescent background with examples of a large (alpha) and small (non-alpha) somas RGCs indicated by asterisks. Two-photon Z-projection images of alpha (<b>B</b>) and non-alpha (<b>C</b>) RGCs following internal whole cell dialysis with pipette filling solution that contains 140 uM OGB-1. Scale bars 30 and 15 um, respectively. Spike train rasters recorded from alpha (<b>D</b>) and non-alpha (<b>E</b>) RGCs and corresponding auto-correlograms (black traces) based on 166 s records, 2 ms bin widths. Red traces are auto-correlograms using shuffled spike trains (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086253#s2" target="_blank">Methods & Materials</a>). Insets show auto-correlograms on expanded time scales to illustrate reduced spike probability during a ∼5 to 10 ms refractory period following a spike.