The effect of Pam3CSK4 on the expression of TLR1, 2, MyD88, and the negative regulatory molecules.

<p>(A) TLR1, 2, and MyD88 gene expression. THP-1 cells were treated with the indicated concentrations of Pam3cks4 for 24 h. The gene expression of TLR1, 2, and MyD88 was detected by RT-PCR. β-actin gene expression was detected as loading controls. (B) IRAK-M, ST2, SOCS1, SIGIRR gene expression. (C, D) A20 gene expression. THP-1 cells were treated with the indicated concentrations of Pam3CSK4 for 24 h (C) or with 1 µg/ml Pam3CSK4 for the indicated time periods (D). A20 gene expression was detected by RT-PCR. β-actin gene expression was detected as loading controls. (E, F) A20 protein expression. THP-1 cells were treated with the indicated concentrations of Pam3CSK4 for 24 h (E) or with 1 µg/ml Pam3CSK4 for the indicated time periods (F). A20 proteins were detected by western blot. β-actin proteins were detected as loading controls.