Time-dependent cleavage of the D-RNA target with the hammerhead Spiegelzyme (heterochiral complex).

<p>The incubation of 0.2 µM target fluorescein-labelled D-RNA, with 2 µM or 0.02 µM hammerhead Spiegelzyme for single <b>(A)</b> and multiple (<b>B</b>) turnover reactions. The reactions were carried out in 50 mM Tris-HCl, pH 7.5, buffer containing 10 mM MgCl₂ at 37°C and different incubation times as indicated. Control reaction was carried out with target D-RNA alone in buffer for 256 min (lane C). Reaction products were separated by 20% polyacrylamide gel electrophoreses containing 7 M urea and analysed by a Fuji Film FLA 5100 phosphoimager<b>. (C)</b> A plot of single (squares, black solid line) and multiple (circle, grey broken line) turnover reactions. The time of reaction is shown in logarithmic scale.