Incubation of D- and L-RNA targets with D-and L-DNAzymes.

<p>Lanes 1 and 2: D- and L-RNA targets (0.2 µM) incubated in 50 mM Tris-HCl buffer, pH 7.5, and 10 mM MgCl₂. Lanes 3 and 4 incubation of D- RNA targets (0.2 µM) with D-and L-DNAzyme (2 µM) in the presence of 10 mM MgCl₂, lanes 5 and 6 incubation of L-RNA target (0.2 µM) with D-and L-DNAzyme (2 µM) in the presence of 10 mM MgCl₂. All incubations were carried out for 5 h at 37°C. The arrow shows products of hydrolysis as expected when the RNA target is hydrolyzed by the Spiegelzymes as indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086673#pone-0086673-g008" target="_blank">Figure 8</a>. Separation of reaction products was accomplished by 20% polyacrylamide gel electrophoresis and 7 M urea.