ER-stress-related cell signaling is suppressed in Ad-DHCR24-infected N2A cells.

<p>N2A cells were infected with the indicated adenoviruses for 2 days, and then exposed to TM for various lengths of time. Whole cell lysates (50 µg protein/lane) were subjected to western blot analysis using anti-phospho-PERK (T981), anti-phospho-p38 (T180/Y182), anti-phospho-JNK (T183/Y185), anti-CHOP or anti-actin antibody. Positive bands were visualized using the ECL method. Representative results are shown in panel A. The phosphor-PERK, phosphor-p38 and phosphor-JNK levels were normalized to actin levels, and are expressed as percentage relative to the maximal level in the cells infected with Ad-LacZ. n = 3, Mean ± S.D., *: p<0.05 vs. the levels in cells infected with Ad-LacZ.