Collinearity of the five completed Planctomycetaceae genomes.

<p><i>P. limnophilus</i> was set as the reference genome. Different colored lines in each genome indicate its similarity profile. The height of the similarity profile corresponds to the average level of conservation in that region of the genome sequence and was calculated to be inversely proportional to the average alignment column entropy over a region of the alignment. Parts of the similarity plot which are colored mauve are conserved among all the five genomes. The other colors shows the similarity of segments not in all the five genomes, but they are too small to notice in a whole-genome view. The similarity profile above the centerline of the aligned region is in the forward orientation relative to the first genome sequence, and profiles below the centerline indicate regions that align in the inverse orientation. Areas that are completely white were not aligned and probably contain sequence elements specific to a particular genome. The Mauve software was used for this analysis <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086752#pone.0086752-Darling1" target="_blank">[55]</a>. The seed (minimum match size) we used was 15 mer.