Inflammatory cell accumulation in the BAL fluid.

<p>BAL fluid was obtained from the lungs of mice following the induction of allergic asthma. Cells were isolated by centrifugation and stained with Diff-Quik stain reagent. Cell numbers were determined using a light microscope to count cells in at least five squaresof a hemocytometer after excluding dead cells using Trypan blue. Total leukocytes (A), macrophages (B), eosinophils (C), neutrophils (D) and lymphocytes (E) from the BAL fluid were counted. Normal control mice treated with PBS only (NC), CKA-challenged mice treated with PBS (CKA), CKA-challenged mice treated with 10 mg/kg of Montelukast (MK), CKA-challenged mice treated with 1 mg/kg of PPY (PPY 1), CKA-challenged mice treated with 2 mg/kg of PPY (PPY 2) and CKA-challenged mice treated with 10 mg/kg of PPY (PPY 10). The data are shown as the mean ± S.E.M. Statistical analysis was conducted by one-way ANOVA followed by the Newman-Keuls Multiple Comparison test (significantly different from NC, <i>*P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001; significantly different from CKA, <i>#P</i><0.05, ##<i>P</i><0.01, ###<i>P</i><0.001, n = 6).