Inhibition of p53 signaling can partially rescue <i>fan</i> mutant craniofacial defects.

<p>(A–B) Whole mount IHC analysis of p53 expression in wild type (A) and <i>fan</i> mutants (B). (C, D) qRT-PCR analysis of <i>d113p53</i> (C) and <i>mdm2</i> (D) gene in wild type and <i>fan</i> mutants at 24, 48 and 72 hpf. (E–G) Bright field images of live 3 dpf wild type (E), <i>fan</i> mutant (F), and <i>fan</i> mutant injected with p53MO (G). (H) Percent zebrafish exhibiting normal eye development in uninjected (Uninj), control MO (CMO), and p53MO injected embryos. (I–K) TUNEL stained and sectioned wild type (WT) (I), <i>fan</i> mutant (J), and <i>fan</i> mutant injected with p53 MO (K). Arrows indicate apoptotic cells. (L) Statistical analyses revealed significantly reduced apoptosis in <i>fan</i> mutants injected with p53MO. (P value<0.01).