Vessel acquisition and reconstruction in the whole mouse brain.

<p>A: The dorsal view of the gelatin-Indian ink perfused brain; after embedding with Spurr resin and imaging using MOST to acquire the three-dimensional cerebrovascular dataset, five 100-µm thick coronal planes were selected and MIP-reconstructed in the olfactory bulb (B), frontal (C), hippocampus (D), midbrain (E) and cerebellum (F); the results showed desirable perfusion in the whole brain, and no blank areas were found in the brain entity. G: ROI in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088067#pone-0088067-g001" target="_blank">Figure 1D</a>; the capillary network was continuous, and no gaps were found. H: The left view of the whole brain; several major vessels were marked. I: The top view of the whole brain. cc: corpus callosum; hf: hippocampus; th: thalamus. B–F: Bar = 1 mm; G: Bar = 200 µm, H: Bar = 1 mm.