Changes in the expression of ethylene (ET) metabolism and signaling genes in response to after-ripening.

<p>Simplified ET metabolic and signaling pathway in plants (A). Fold changes (log₂-scale) in the expression of ET metabolism (B) and signaling (C) related probesets between after-ripened (AR) and dormant (D) seeds in both dry (AR-0/D-0) and imbibed states (AR-12/D-12 and AR-24/D-24), and between AR seeds imbibed without and with ABA (AR-24/AR-24+ABA). The log₂ fold change values are shown by the negative and positive numbers on the bar, and the color scale shows upregulation (red) and downregulation (olive green) of the respective probesets. Fold changes (linear-scale) in expression and the associated <i>P</i> values are presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087543#pone.0087543.s002" target="_blank">Table S2</a>. Data are means of 3 independent biological replicates. Abbreviations: <i>ACS</i>, <i>1-aminocyclopropane-1-carboxylic acid synthase</i>; <i>ACO</i>, <i>1-aminocyclopropane-1-carboxylic acid oxidase</i>; <i>RAN</i>, <i>responsive-to-antagonist</i>; <i>RTE</i>, <i>reversion-to-ethylene sensitivity</i>; <i>ETR</i>, <i>ethylene response</i>; <i>ERS</i>, <i>ethylene response sensor</i>; <i>CTR</i>, <i>constitutive triple response</i>; <i>TPR</i>, <i>tetratricopeptide repeat</i>; <i>EIN2</i>, <i>ethylene insensitive 2</i>; <i>ECIP</i>, <i>EIN2 c-terminus interacting protein</i>; <i>EIN5</i>, <i>ethylene insensitive5</i>; <i>EIN3</i>, <i>ethylene insensitive 3</i>; <i>EBF</i>, <i>EIN3-binding F box protein</i>; <i>EER</i>, <i>enhanced ethylene response</i>; <i>ERF</i>, <i>ethylene-responsive element binding factor</i>; <i>MPK3</i>, <i>mitogen-activated protein kinase 3</i>; <i>MPK6</i>, <i>mitogen-activated protein kinase 6</i>.