Validation of candidate genes from ChIP-seq via QT-PCR in <i>STAT5A</i> and <i>STAT5B</i> knock-down human CD4⁺ T cells.

<p>The expression levels were compared <i>STAT5A</i> KD or <i>STAT5B</i> KD CD4⁺ T cells with control CD4⁺ T cells. Genes regulated similarly by both STAT5A and STAT5B are <i>SGK1</i>, <i>GTF2H5</i> and <i>SLC22A5</i>. Genes regulated specifically by STAT5A are <i>NDRG1</i>, <i>DNAJC6</i>, <i>ST3GAL1</i>, <i>SAMD4A</i>, <i>SSH2</i>, <i>MAP3K5</i> and <i>BCL2L1</i>. Genes regulated specifically by STAT5B are <i>DOCK8</i>, <i>SNX9</i>, <i>SKAP1</i>, <i>TNFSF10</i>, <i>FOXP3</i>, <i>IL2RA</i> and <i>UGCG</i>. Data is presented as mean ± SEM. *, P<0.01; **, P<0.001. There were no statistical differences if not annotated. <b>Abbreviations</b>: <i>SGK1</i>, serum/glucocorticoid regulated kinase 1; <i>GTF2H5</i>, general transcription factor IIH, polypeptide 5; <i>BCL2L1</i>, BCL2-like 1; <i>SLC22A5</i>, solute carrier family 22 (organic cation/carnitine transporter), member 5; <i>CDKAL1</i>, CDK5 regulatory subunit associated protein 1-like 1; <i>DNM2</i>, dynamin 2; <i>DUSP5</i>, dual specificity phosphatase 5; <i>MBP</i>, myelin basic protein; <i>ARL4C</i>, ADP-ribosylation factor-like 4C; <i>NDRG1</i>, N-myc downstream regulated 1; <i>DNAJC6</i>, DnaJ (Hsp40) homolog, subfamily C, member 6; <i>ST3GAL1</i>, ST3 beta-galactoside alpha-2,3-sialyltransferase 1; <i>SAMD4A</i>, sterile alpha motif domain containing 4A; <i>SSH2</i>, slingshot protein phosphatase 2; <i>MAP3K5</i>, mitogen-activated protein kinase kinase kinase 5; <i>CBS</i>, cystathionine-beta-synthase; <i>PPP2R2B</i>, protein phosphatase 2, regulatory subunit B, beta; <i>DOCK8</i>, dedicator of cytokinesis 8; <i>SNX9</i>, sorting nexin 9; <i>SKAP1</i>, src kinase associated phosphoprotein 1; <i>PTGER1</i>, prostaglandin E receptor 1 (subtype EP1), 42 kDa; <i>DIDO1</i>, death inducer-obliterator 1; <i>TNFSF10</i>, tumor necrosis factor (ligand) superfamily, member 10; <i>FOXP3</i>, forkhead box P3; <i>IL2RA</i>, interleukin 2 receptor, alpha; <i>UGCG</i>, UDP-glucose ceramide glucosyltransferase; <i>LNPEP</i>, leucyl/cystinyl aminopeptidase; <i>PPP1R3F</i>, protein phosphatase 1, regulatory subunit 3F.