Early activation of Akt and ERK1/2 by ROS in Intralipid®-treated hearts.

<p>Panel A: p-Akt to total Akt immunoblots from tissue samples collected 3 min after reperfusion. Panel B: p-STAT3 to total STAT3 immunoblots from same tissue samples. Panel C: Akt activity measurements. Panel D: p-ERK1/2 to total ERK immunoblots from same tissues. IR, untreated hearts exposed to 15 min of ischemia and 3 min of reperfusion (IR). IR/IL, hearts exposed to IR and 1% Intralipid® at the onset of reperfusion. MPG, N-(2-mercaptopropionyl) glycine (10 µM) added at the onset of reperfusion. Data are mean (SEM). N = 6 hearts in each group.