Feedback

Intralipid®-induced formation of ROS in cardiac fibers collected 3 min after reperfusion.

  • Phing-How Lou (198977)
  • Eliana Lucchinetti (517250)
  • Liyan Zhang (322168)
  • Andreas Affolter (517251)
  • Marcus C. Schaub (517252)
  • Manoj Gandhi (517253)
  • Martin Hersberger (118533)
  • Blair E. Warren (517254)
  • Hélène Lemieux (326079)
  • Hany F. Sobhi (517255)
  • Alexander S. Clanachan (517256)
  • Michael Zaugg (517257)
Open link
Publication date
January 2014
Icon DOI
10.1371/journal.pone.0087205.g003

Abstract

<p>Panel A: Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) emission capacity from mitochondria as determined by Amplex Red assay. *significantly different from IR. Panel B: Loss of aconitase activity in Intralipid®-treated hearts (P<sub>ANOVA</sub> = 0.002). IR, untreated hearts exposed to 15 min of ischemia and 3 min of reperfusion (IR). IR/IL, hearts exposed to IR and 1% Intralipid® at the onset of reperfusion. Basal, without substrates. +subst, with added substrates. AER, hearts with time-matched aerobic perfusion. Data are mean (SEM). N = 6 hearts in each group.</p

Extracted data

We use cookies to provide a better user experience.
Data Protection