Spore production and germination in <i>C. difficile</i> biofilms.

<p><b>A)</b> For each strain, cells were harvested from 6-day old biofilms and the numbers of spores and vegetative cells counted using phase-contrast light microscopy. Spore % was calculated as the # of spores/# of spores+# of vegetative cells. <b>B)</b> For each strain, spores were harvested from 6-day old cultures on Columbia blood agar plates or biofilms on T soy agar plates, treated with 1% taurocholate, and the numbers of germinated and ungerminated spores counted using phase-contrast light microscopy. Percentage of germinated spores was calculated as # germinated spores/# germinated spores+# ungerminated spores.