Dorsomorphin inhibits phosphorylation of Smad1/5.

<p>Bmp signalling activity was quantified by measuring phosphorylation of Smad 1/5 at the late gastrula stage. Embryos were exposed to 100 µM from 32-cell as well as 100 and 200 µM DM from late blastula. These were then frozen at the late gastrula stage and used for Western Blotting. <b>A:</b> Quantification of densitometry results obtained from 3 independent experiments (Mean ± SE), normalised to total Smad and indicated as fold increase over the resting control condition. All 3 treatments were significantly different from the control as indicated by asterisks (P<0.001), but no significant differences were observed between the treatments. <b>B:</b> Representative Western Blot of 3 independent experiments showing the levels of total Smad1/5/8 and phospho-Smad1/5 at late gastrula from the dose and stage specific treatments. These data demonstrate that all 3 treatments equally suppress phospho-Smad1/5 by late gastrula.