Analysis of spleen and brain lymphocytes of cerebral malaria developing <i>P. berghei</i> ANKA-infected WT vs Ly49E KO mice.

<p>Freshly isolated spleen (A–C) or brain (D–F) NK (CD3⁻NK1.1⁺), NKT (CD3^dimNK1.1⁺), CD4⁺ T (CD3⁺CD4⁺), CD8⁺ T (CD3⁺CD8⁺) and γδ T cells (CD3⁺TCRγδ⁺) from non-infected WT and Ly49E KO mice or cerebral malaria developing <i>P. berghei</i> ANKA-infected WT and Ly49E KO mice (as indicated in the figure) were analyzed by flow cytometry. The percentage of these lymphocyte populations are shown in A and D. CXCR3 and CD69 expression was analyzed within each lymphocyte population (B and E). NK receptor expression was determined in NK and NKT cells (C and F). Data represent the results of 2 independent experiments (WT, n = 8; KO, n = 11) and are shown as mean ± SEM. Statistical analysis was performed using the 2-tailed Mann Whitney test. * represent a significant difference with p<0.05.