TLRs and NLRs are not involved in <i>Leptospira</i>-induced fibrosis.

<p>WT and Myd88ko mice infected with 2×10⁶<i>L. interrogans</i> Fiocruz were sacrificed at 15 days p.i. (A) Bacterial loads in 100 µl of urine. (B) Inflammation score in kidneys. (C) Red Sirius staining (C, left panel) and mRNA expression of ACTA-2, fibronectin and Mmp2, in the infected WT and Myd88ko mice kidneys (C, right panel). Data are means ± SD from WT mice (n = 3) and MyD88ko mice (n = 5). The images in C are representative of three separate experiments. (D) Percentage of Red Sirius staining in kidneys from WT mice, TLR3ko, Casp1ko and double Nod1/2ko mice infected with 2×10⁸<i>L. interrogans</i> Fiocruz and sacrificed at day-15 p.i. (n = 4 per genotype group). Three naïve kidneys of each genotype were used as controls. *P<0.05; **P<0.01 between groups.