<p>(<b>A</b>) Survival of WT, <i>Δdot1, Δyca1</i> and <i>Δdot1Δyca1</i> cells was determined by clonogenicity during chronological aging. Data represent mean ± SD (<i>n</i> = 3). (<b>B</b>) Integrals under the life span curves were determined. Data represent mean ± SD (<i>n</i> = 3, **P<0.01). (<b>C</b>) ROS accumulation, DNA fragmentation, and phosphatidylserine exposure on the cell membrane in WT and <i>Δdot1</i> cells after 5 days in culture determined by DHE, TUNEL and Annexin V/PI staining, respectively. Scale bars, 10 µm. (<b>D</b>) DHE-positive WT, <i>Δdot1</i>, <i>Δyca1</i> and <i>Δdot1Δyca1</i> cells were quantified after 5 days in culture using flow cytometry. In each experiment, 10.000 cells were evaluated. Data represent mean ± ...
<p>(<b>A</b>): Cumulative population doublings of aMSCs and yMSCs during the first 80 days of cultur...
<p>Accumulation of ROS in cells under nitrogen starvation <b>(A)</b> and <b>(B)</b> during aging cul...
<p>(<b>A</b>) FA lifespan (expressed as the relative number of surviving FAs) and average normalized...
<p>(<b>A</b>) Survival of wild-type (WT), <i>Δyca1</i>, <i>Δset1</i> and <i>Δset1Δyca1</i> cells was...
<p>(<b>A</b>) Survival of WT, <i>Δyca1</i>, <i>Δdot1</i>, <i>Δset1</i>, <i>Δset1Δdot1</i> and <i>Δse...
<p>(<b>A</b>) Survival of WT, <i>Δset1</i>, <i>Δnuc1</i> and <i>Δset1Δnuc1</i> cells was determined ...
<p>(A) Cell counts showing YFP (“loser” cells) cells first expand, then decline, in the presence of ...
The DNA-damage response (DDR) initiates cellular processes that ca lead to DNA repair, senescence or...
BACKGROUND: To study the apoptotic process in time, we used the following flow cytometric (FCM) tech...
The DNA-damage response (DDR) initiates cellular processes that can lead to a difficult choice: DNA ...
The DNA-damage response (DDR) is crucial for the maintenance of DNA integrity and genomic stability...
<p>(<b>A</b>) Growth curve of 3 independent MRC-5 fibroblast cell lines (control with no quiescence ...
<p>Cultures of MCF10A acini were fed on days 4 and 8 (indicated by the arrows) in the absence (squar...
<p>HCT116 cells transfected with siRNA directed against <i>DOT1L</i> or scrambled siRNA control thre...
<p>A: Legend for Annexin V/ Propidium Iodide flow plots (left) and representative flow plots of WT a...
<p>(<b>A</b>): Cumulative population doublings of aMSCs and yMSCs during the first 80 days of cultur...
<p>Accumulation of ROS in cells under nitrogen starvation <b>(A)</b> and <b>(B)</b> during aging cul...
<p>(<b>A</b>) FA lifespan (expressed as the relative number of surviving FAs) and average normalized...
<p>(<b>A</b>) Survival of wild-type (WT), <i>Δyca1</i>, <i>Δset1</i> and <i>Δset1Δyca1</i> cells was...
<p>(<b>A</b>) Survival of WT, <i>Δyca1</i>, <i>Δdot1</i>, <i>Δset1</i>, <i>Δset1Δdot1</i> and <i>Δse...
<p>(<b>A</b>) Survival of WT, <i>Δset1</i>, <i>Δnuc1</i> and <i>Δset1Δnuc1</i> cells was determined ...
<p>(A) Cell counts showing YFP (“loser” cells) cells first expand, then decline, in the presence of ...
The DNA-damage response (DDR) initiates cellular processes that ca lead to DNA repair, senescence or...
BACKGROUND: To study the apoptotic process in time, we used the following flow cytometric (FCM) tech...
The DNA-damage response (DDR) initiates cellular processes that can lead to a difficult choice: DNA ...
The DNA-damage response (DDR) is crucial for the maintenance of DNA integrity and genomic stability...
<p>(<b>A</b>) Growth curve of 3 independent MRC-5 fibroblast cell lines (control with no quiescence ...
<p>Cultures of MCF10A acini were fed on days 4 and 8 (indicated by the arrows) in the absence (squar...
<p>HCT116 cells transfected with siRNA directed against <i>DOT1L</i> or scrambled siRNA control thre...
<p>A: Legend for Annexin V/ Propidium Iodide flow plots (left) and representative flow plots of WT a...
<p>(<b>A</b>): Cumulative population doublings of aMSCs and yMSCs during the first 80 days of cultur...
<p>Accumulation of ROS in cells under nitrogen starvation <b>(A)</b> and <b>(B)</b> during aging cul...
<p>(<b>A</b>) FA lifespan (expressed as the relative number of surviving FAs) and average normalized...