Plasmacytoid DC produce IFNα in both lymphoid and mucosal compartments.

<p>Dotplot showing IFNα intra-cellular staining in gated pDC (CD45⁺HLA-DR⁺lin⁻CD123⁺) in different tissues. Cells were labeled <i>ex vivo</i> on fresh cells after 30 min incubation in 10 µg/mL brefeldin A in the absence of any stimulation. Data for two macaques sacrificed on day 10 p.i. and one uninfected control are shown. Mononuclear cells from BM, spleen, peripheral LN, mesenteric LN, ileum, and colon were extracted for FACS analysis. Frequencies of IFNα-pDC are indicated in bold and # indicates the number of pDC recorded for each file.