CXCR7 silencing decreases MOLT4 and Jurkat cell migration.

<p>Cell migration toward either RPMI with 0.1% BSA and RPMI or 0.1% BSA containing CXCL12 (200 ng/mL) used as negative control and chemoattractant, respectively. After 4 h, the number of migrated cells was counted and was expressed as a percentage of the input, i.e., the number of cells applied directly to the lower compartment in parallel wells. The migration of cells was normalized to 100% +/− sd of triplicates. (A) The CXCR7 silencing resulted in significant changes in MOLT4 chemotactic response (<i>P = 0.0159</i>). The inhibition of CXCR4-dependent chemotaxis by its antagonist AMD3100 (1.25 µg/mL) promoted a similar effect (<i>P = 0.0159</i>). Moreover, the silencing of CXCR7 plus the treatment with AMD3100 exhibited a synergistic effect in cell chemotactic capacity (<i>P = 0.0086</i>). (B) The same effect was observed with Jurkat cells. The CXCR7 silencing (<i>P = 0.0366</i>) or the inhibition of CXCR4-dependent chemotaxis by its antagonist AMD3100 (<i>P = 0.019</i>) reduced Jurkat chemotactic response. The simultaneous silencing of CXCR7 and treatment with AMD3100 also exhibited a synergistic effect upon cell chemotactic capacity (<i>P = 0.0191</i>); <i>Mann-Whitney</i> test.