Higher expression of CXCR7 in T-acute lymphoid leukemia lines MOLT4 and Jurkat.

<p>A) Western blot analysis of CXCR7 protein levels in myeloid (U937, P39, K562 and KG-1), B-lymphoid (Daudi, Raji) and T-lymphoid (MOLT4 and Jurkat) cell lines. Total cell extracts were blotted with antibodies against CXCR7 (42 kDa), CXCR4 (42 kDa) or β-actin (42 kDa), as a control for equal sample loading, and developed with the ECL Western Blot Analysis System. CXCR7 protein was detectable in all acute leukemia cell lines; however CXCR7 was more expressed in the T-acute lymphoid cell lines MOLT4 and Jurkat when compared to other cell lines. CXCR4 proteins levels were homogeneous in all cell lines analyzed. B) Quantitative expression of <i>CXCR7</i> mRNA in leukemic cells lines. mRNA expression levels of <i>CXCR7</i> were normalized by <i>HPRT</i> and <i>GAPDH</i> endogenous control. <i>CXCR7</i> mRNA was more expressed in T-acute lymphoid cell lines MOLT4 and Jurkat when compared to other cell lines.