Effect of Nur77 on hepatic lipid deposition in apoE^−/− mice fed a high-fat/high cholesterol.

<p>(A, B and C) ApoE^−/− mice were randomized into eight groups–Baseline (fed a standard chow diet), Control (fed a high-fat/high diet), Vehicle (fed a high-fat/high diet and treated with PEG400: Tween 80, 4∶1), Csn-B (fed a high-fat/high diet and treated with Csn-B, 10 mg/kg body weight), LV-Mock (fed a high-fat/high diet and injected via the tail vein with control lentivirus), LV-Nur77 (fed a high-fat/high diet and injected via the tail vein with lentivirus encoding mouse Nur77), Si-Mock (fed a high-fat/high diet and injected via the tail vein with control lentivirus) and Si-Nur77 (fed a high-fat/high diet and injected via the tail vein with lentivirus encoding mouse Nur77). (A) a, Western blot analysis of the protein expression of Nur77 in mouse liver. b, Liver cryo-sections immunohistochemically stained. Original magnification: 200X. c, Liver cryo-sections were stained with Oil Red O and hemotoxylin. Original magnification: 100X. d, Hepatic lipid deposition was analyzed in apoE^−/− mice. (n = 5 per group). Values are the percent of the total lesion area stained and are expressed as mean ± S.D. * <i>p</i><0.05; ** <i>p</i><0.01; *** <i>p</i><0.001. (B) a, b and c, gene expression was measured by real-time quantitative PCR. Data represent the mean values ± S.D. n = 5, performed in triplicate. * <i>p</i><0.05 vs. vehicle group, ^#<i>p</i><0.05 vs. LV-Mock group, and <i>p</i><0.05 vs. si-Mock group. (C) Liver triglyceride levels were measured. Values are the mean ± S.D. n = 5, performed in triplicate. * <i>p</i><0.05; ** <i>p</i><0.01; *** <i>p</i><0.001.