Detection of irisin in bovine plasma and murine serum.

<p>Western blot of plasma from bulls with high (H) or low (L) muscularity with an antibody against full-length irisin (upper panel). M: murine serum. x: empty lanes. A band specific for irisin was observed only in murine serum and is marked by a red arrow. The blot was stripped and re-incubated with the antibody against the C-terminus of FNDC5 (lower panel). A specific band was detected neither in bovine plasma nor in murine serum.