Characterization of the FNDC5 antibody.

<p>Western blot of mouse serum (1, 2), murine M. quadriceps femoris (3, 4), bovine plasma (5, 6), bovine M. longissimus (7), and bovine M. semitendinosus (8) with antibody against C-terminus of FNDC5 (left panel). The specific band of ∼25 kDa is marked by a red arrow. The right panel shows a parallel blot where the antibody was blocked with the recombinant antigen peptide prior to incubation. This antibody was raised against aa 149–178 of human FNDC5 corresponding to aa 143–171 in cattle (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088060#pone-0088060-g003" target="_blank">Figure 3</a>, transcript T1–T1).