Characterization of the irisin antibody.

<p>Western blot of bovine plasma (1, 2), LM (3), and SM (4) with an antibody against full-length irisin. Recombinant irisin (5) was used as positive control (red arrow; left panel). The right panel shows a parallel blot where the antibody was blocked with recombinant irisin prior to incubation. The antibody was raised against aa 32–143 of human FNDC5 (corresponding to aa 25–136 in cattle; <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088060#pone-0088060-g003" target="_blank">Figure 3</a>, transcript T1–T1).