Bovine FNDC5 protein variants and antibody binding.

<p>(<b>A</b>) Deduced amino acid sequences for different transcripts and transcript fragments identified in this study. Putative signal peptides are underlined and the irisin peptide is marked in bold letters. Amino acids 25-204 in transcript T1–T1 are identical with murine and human amino acid sequences. (<b>B</b>) Schematic representation of antibody binding in the bovine FNDC5 protein and irisin peptide, respectively. The antibodies I, II, and III are further described in Materials and Methods. Annotation of functional domains and irisin was borrowed from the murine protein <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088060#pone.0088060-FerrerMartinez1" target="_blank">[1]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088060#pone.0088060-Bostrom1" target="_blank">[3]</a>. SP: signal peptide; H: hydrophobic domain; C: C-terminal domain. Numbers refer to amino acids of bovine full length transcript T1–T1 (see A).