Ki-67 proliferative activity in the left ventricle.

<p>A, B, C, D, Low Ki-67 proliferative activity in normal left ventricle showing nuclear staining of Ki-67 in one interstitial cell (arrow head). E, F, G, 20 minutes MI; I,J, K, 30 minutes MI; M,N,O 60 minutes MI; Q,R,S, 4-hour MI, Low Ki-67 proliferative activity: showing nuclear staining of Ki-67 in few endothelial cells (arrow head) in left ventricle. U, V, W, 24-hour MI, high Ki-67 proliferative activity showing nuclear staining of Ki-67 in a large number of endothelial cells (arrow head) in the infarction area of left ventricle. H, L, P, T, X, showing faint nuclear staining of Ki-67 in one endothelial cell in 20 minutes, 30 minutes, 60 minutes, 4-hours and 24 hour sham-operated left ventricle. A, E, I, M, Q, U are stained by Streptavidin-Biotin immunoperoxidase method. The others are stained by Alexa Fluor 488 immunofluorescent technique.