AntagomiR-mediated inhibition of miR-27a enhances endogenous Mstn expression and function <i>in vivo</i>.

<p>(A) qPCR analysis of <i>Mstn</i> mRNA expression in TA muscle isolated from WT mice (n = 3) 8 days post intramuscular injection of either AntagomiR Neg or AntagomiR-27a. <i>p</i><0.05 (*). (B) Representative images of H&E stained AntagomiR Neg and AntagomiR-27a injected TA muscle from WT mice. Scale bars = 100 µm. (C) Graph showing average myofiber CSA (µm²) in AntagomiR Neg and AntagomiR-27a injected TA muscle from WT mice. Average myofiber area was calculated from 10 random images per coverslip (n = 3). (D) Frequency distribution of myofiber area (µm²) in AntagomiR Neg and AntagomiR-27a injected TA muscle from WT mice as calculated from 10 random images per coverslip (n = 3). (E) <i>Left</i>: Representative merged immunofluorescence image showing a Pax7⁺ cell (Green; white arrowhead) in an AntagomiR Neg injected TA muscle cross section from WT mice. Sections were also stained for Laminin (Red) and nuclei were counterstained with DAPI (Blue). Scale bar = 10 µm. <i>Right</i>: Graph showing the number of Pax7⁺ cells in AntagomiR Neg and AntagomiR-27a injected TA muscle from WT mice. Bars represent mean number ± S.E.M of Pax7⁺ cells, per 100 myofibers, from 3 sections each collected from AntagomiR Neg and AntagomiR-27a injected WT mice (n = 3). <i>p</i><0.01 (**). (F) Graph showing the number of MyoD⁺ cells in AntagomiR Neg and AntagomiR-27a injected TA muscle from WT mice. Bars represent mean number ± S.E.M of MyoD⁺ cells, per 100 myofibers, from 3 sections each collected from AntagomiR Neg and AntagomiR-27a injected WT mice (n = 3). <i>p</i><0.001 (***).