Characteristics of cells <i>in vitro</i> and evidence of cells seeded on the scaffold placed in the skull critical size defect.

<p>Shape and appearance of EPCs and MSCs obtained from rats in (A) and (B), respectively. Dil-uptake as a marker of endothelial differentiation is exemplarily shown for EPCs seeded on BG20 (C), the calcium deposition as measured by von Kossa staining revealed osteogenic differentiation of MSCs (black areas, D). Adherence of MSCs and EPCs one hour after seeding to the BG20 scaffold (E). MSCs and EPCs can be differentiated by size. Position of scaffold loaded with cells (arrow) in the skull critical size defect of the rat (F). Scale bars: 100 µm (A, B), 200 µm (D), 10 µm (E).