Comparative study between MSC, EWS cell lines and EWS samples.

<p>(A) Hierarchical unsupervised cluster analysis showed two different clusters, one consisting of all EWS cell lines and the other of all MSC samples. Control was performed using Fluorescence minus one. The following markers: CD105, CD34, CD45 and CD90 were used in every tube in order to diminish the auto-fluorescence presented by MSC, therefore these were not contemplated in this analysis, and were validated in frozen tissue from EWS samples. (B) IHC studies in frozen tissue from EWs samples. Upper panel represents EWS typical morphology as well as CD99 strong expression. Middle panel shows that a strong expression of CD90 and positivity for CD105. Finally, lower panel shows that EWS present negative expression of both CD45 and CD34.