Disruption of <i>bck1</i>, <i>mkk1</i> and <i>slt2</i> in <i>B. bassiana</i> inreases osmosensitivity and affects HOG pathway.

<p>(<b>A</b>) Relative growth inhibition (RGI) of fungal colonies after 8-day growth at 25°C on 1/4 SDAY supplemented with NaCl (1 M) and sorbitol (1.2 M). Asterisked bars in each group differ significantly from those unmarked (Tukey's HSD, <i>P</i><0.05). Error bars: SD from repeated assays of three replicates. (<b>B</b>) Images of the fungal colonies grown under osmotic stress. (<b>C</b>) Western blots for the existence (lower) and phosphorylation signal (upper) of Hog1 in protein extracts probed with rabbit anti-p38 and anti-phospho-p38 MAPK antibodies respectively. The proteins samples were extracted from the SDB cultures (hyphal cells) of different strains stressed with NaCl (1 M) for 90 min at 25°C.