The macrophage-biomaterial interface: a proteomic analysis of the conditioned medium environment

well as extracellular microenvironments. However, proteomics has not been extensively applied to examine cell-material interface dynamics or the conditioned medium (CM) that ensues. Monocyte-derived macrophages (MDM) are of particular interest as they are the predominant cell type contributing to chronic inflammation at long-term implant sites. The current study compares the CM generated when MDM were adherent to tissue culture polystyrene (TCPS) or a model polycarbonate-urethane (PCNU). Two-dimensional electrophoresis and either matrix-assisted laser desorption/ionization time-of-flight mass spectrometry or liquid chromatography Fourier transform mass spectrometry were used for protein identification to begin to assess how the CM is altered when MDM are in contact with a degradable PCNU material relative to a typical tissue culture surface