Increased miR-130b expression observed in CRC specimens.

<p><i>A</i>, Heat map diagram of the 31 significantly elevated miRNAs in colorectal cancer tissues compared to matched adjacent control tissues from 3 patients (P1, P2, and P3). The adjacent control tissues are referred to N1, N2 and N3. The tumors are referred to T1, T2 and T3. MiRNAs are shown in rows. Samples are shown in columns. Red in the color bar indicates higher expression and blue indicates lower expression. MiR-130b (<i>Red Arrow</i>) is one of significantly upregulated miRNAs. <i>B</i>, Relative expressions of miR-130b (normalized to U6) in tumors over adjacent control tissues (T/N) from P1, P2, and P3, determined by qRT-PCR (mean±s.d.; n = 3). <i>C</i>, <i>Upper panel</i>: Schematic diagram of a lentiviral pLVX-IRES-Hyg vector containing the primary microRNA 130b (Lenti-miR-130b). <i>Lower panel</i>: Relative expressions of miR-130b (normalized to U6) in SW480 cells that stably expresses miR-130b (Lenti-miR-130b) or control (Lenti-control), examined by qRT-PCR (mean±s.d.; n = 3). The four colonies of Lenti-miR-130b cells are referred to 1,2,3,4.