Positive expression of OPN and αvβ3 protein in hepatocellular carcinoma (HCC).

<p>(A) HE staining. The nuclei of tumor cells are large, and have atypia, which are different from those of adjacent non-tumorous hepatocytes (HE 20×10). (B) Ki-67 immunohistochemical staining. Ki-67 expression is observed in the nucleus and is visualized as brown-yellow staining. Ki-67 index is higher in tumor but lower in adjacent non-tumorous hepatic tissues (SP 20×10). (C&D) OPN expression is predominantly observed in the cytoplasm and is visualized as brown-yellow staining. The expression of OPN is negative in adjacent non-tumorous tissue (C: SP 20×10, D: SP 40×10). (E&F) αvβ3 immunohistochemical staining. Expression of αvβ3 is observed predominantly in the membrane or cytoplasm and is visualized as brown-yellow staining. The expression of αvβ3 is negative in the adjacent non-tumorous tissue (E: SP 20×10, F: SP 40×10).