Restoration of ITGB1 expression rescues miR-134-induced suppression of HCC migration and invasion.

<p><b>A</b> and <b>B.</b> Transwell migration assays of (A) SK-HEP-1 and (B) Huh-7 cells stably overexpressing ITGB1. Representative images are shown with the quantification of five randomly selected fields. The bars represent the SEM of three independent experiments. Differences between two groups were analyzed by the Mann-Whitney test. Western blot analysis revealed that the protein expression of ITGB1 increased after infection with the PLVX-ITGB1 (ORF) virus. <b>C.</b> Transwell migration and invasion assays for SK-HEP-1-vector or SK-HEP-1- miR-134 cells were performed after infection with ITGB1 (ORF) or the vector control lentivirus. Transwell migration and invasion data were analyzed by a one-way ANOVA (p<0.01, p<0.01 respectively); differences between two groups were assessed by the Bonferroni post-test. ITGB1 protein levels were determined by Western blot analysis. <b>D.</b> Transwell migration and invasion assays for Huh-7-vector or Huh-7-miR-134 cells were performed after infection with ITGB1 (ORF) or vector control lentivirus. ITGB1 protein levels were determined by Western blot analysis. Transwell migration and invasion data were analyzed by a one-way ANOVA (p<0.01, p<0.01 respectively); differences between two groups were assessed by the Bonferroni post-test.