SMARCA5 cooperates with CTCF on enhancer-blocking activity of <i>H19</i> promoter.

<p><b>A:</b> schematic representation of the reporter constructs pIHLE (without ICR), pIHLME (with mutated CTCF bindng site at the ICR) and pIHLIE (with ICR that can bind CTCF). ICR, insulator of <i>H19</i> gene; H19, promoter of <i>H19</i> gene; luciferase, luciferase gene; Enh, SV40 enhancer; MUT, insulator with mutated CTCF binding sites; kd, protein knock-down. <b>B:</b> Luciferase activity of reporter constructs in HeLa cells. Luciferase acitivity was normalized on Renilla, the pIHLE was set equal to 1. <b>C:</b> HeLa cells depleted for either CTCF or SMARCA5 (using siRNAs) were analyzed for luciferase activity of reporters. Luciferase activity was normalized on non-specific siRNA (Ctrl siRNA) that was set equal to 1, error bars indicate standard errors (SE), t-test (p<0.05 indicated by star).