Conserved intron positions and location of MXE encoded regions.

<p>A) The gene structure alignment was generated with Genepainter <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088111#pone.0088111-Hammesfahr1" target="_blank">[58]</a> by mapping intron positions obtained from the gene structure reconstructions onto the protein multiple sequence alignment. Genepainter requires intron positions not only conserved at the amino-acid level but also at the nucleotide level (codons might be split differently). Hyphens “-” represent coding regions and vertical bars “|” denote intron positions. Common intron positions in the gene structure alignment are conserved down to the nucleotide level. Conserved clusters of MXEs are colour coded and numbered from N- to C-terminus (see legend). The same colour coding and numbering scheme will be used throughout this analysis for all MXEs. Some branch names are given for better orientation. B) The structure of the motor domain of the non-muscle class-II myosin of <i>Dictyostelium discoideum</i> <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088111#pone.0088111-Kliche1" target="_blank">[59]</a> has been used to highlight the regions encoded by alternatively spliced exons. For colouring the regions encoded by MXEs the same colours have been used as for the gene structures in A). The clusters of MXEs not described so far code for the light-green (cluster-3) and the dark-brown (cluster-4) part of the structure.