Effect of of anti-catalytic mAbs to N-domain active center for A and C for B) and from patient # 27 (arrowed in Fig. 1 C) were incubated with mAbs which are anti-catalytic for the N-domain active center - i2H5 and 3A5 (10 µg/ml)[23], [34].

<p>Residual ACE activity was determined with two ACE substrates as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088001#pone-0088001-g001" target="_blank">Fig. 1</a>. We also measured ZPHL/HHL ratio-as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088001#pone.0088001-Danilov4" target="_blank">[20]</a> and in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088001#pone-0088001-g001" target="_blank">Fig. 1 C</a>. Bars highlighted with yellow - samples with values of ACE activity lower than mean ± 2SD for control sample without mAbs. Bars highlighted with red- samples with values of ZPHL/HHL ratio higher than mean ± 2SD for control samples without mAbs. Data presented are mean ± SD of 2–3 independent experiments in duplicates). * p<0.05 vs. control serum.