Critical residues in the active center of the N-domain of mutant ACE.

<p>The RXP407–N-domain co-crystal structure of the N domain of ACE with N-domain specific inhibitor RXP407 - PDB accession code 3NXQ <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088001#pone.0088001-Balyasnikova3" target="_blank">[42]</a>. Key amino acids in the N domain crucial for substrate specificity and ACE inhibition are denoted by somatic ACE numbering and are shown as green sticks. The catalytic zinc ion is shown in slate blue and RXP407 in blue. A water molecule that could possibly be involved in an interaction between the C-terminal amide of RXP407 and Thr358 is given as a small blue sphere. Image generated using PyMOL software and Ser333 has been replaced with a Trp (purple stick representation) using PyMOL Mutagenesis Wizard (v 0.99, DeLano Scientific).