Effect of H₁R and H₄R antagonists on Th2 migration, cytokine secretion and skin inflammation.

<p>(A) FACS analysis of single-cell suspensions from patched skin reveals reduced infiltration of transgenic T-cells into the skin after treatment with H₁R and/or H₄R antagonists (20 mg/kg body weight) compared to vehicle-treated controls. Lymphocytes were gated based on their FSC/SSC characteristics. Depicted are flow cytometric analyses for CD4 and KJ1-26 expression, identifying transgenic OVA-specific T-cells. Experiments were performed independently 2 times; shown are representative stainings of pooled samples, n = 4–5 animals/group. (B) ELISA supernatant analysis for IL-4, IL-13 and IFN-gamma secretion from cells from skin dLNs after OVA-specific restimulation for 3 days compared to vehicle-treated control animals. Columns and error bars represent the mean ± SEM from 2 independent experiments with pooled dLNs (n = 4–5 animals/group) being analyzed. (C) Representative giemsa staining of skin from mice which received Th2 cells, were OVA-patched and treated with H₁R antagonist (20 mg/kg bodyweight) in different magnifications. Arrow indicates mast cell. Scale bar = 200 μm or 50 µm. mast cells enumerated on 10 HPF per section, n = 4 animals/group. ***p<0.001; **p<0.01; ns = not significant.