<i>In vitro</i> and <i>in vivo</i> evidence of MP-mediated horizontal transfer of mRNA into HUVEC and confocal microscopic analysis of engraftment of PKH 26 stained MPs into kidney with I/R injury.

<p>(A) RT-PCR analysis for GFP gene in HUVEC. GFP mRNA was detected only from HUVEC incubated with GFP-MPs, and not from untreated HUVEC. (B) Kidney tissue from I/R injured mice that were administered MPs derived from GFP transfected KMSC positively expressed. As a positive control, GFP-DNA was used and β-actin mRNA was used as a housekeeping gene. (C) The average number of engrafted MPs in mice given intravenous injection of MPs from KMSC. MPs were estimated by observing 20 randomly chosen fields from cortex and corticomedullary junction. Results are expressed as mean ± SE. (D) Confocal laser microscopy confirmed green CellTracker™ labeled MPs colocalized within peritubular capillaries stained with red fluorescent anti-CD31 in the kidney. White arrows indicate green CellTracker™ labeled MPs (upper panel). Yellow arrow indicates the engraftment of green CellTracker™ labeled MPs in peritubular capillaries. (lower panel). White bar represents 20 µm.