LPS-stimulated phosphorylation of MAPKs to induce proinflammatory cytokines.

<p>RAW264.7 cells were plated at 1.0×10⁵ cells/well and cultured in DMEM containing 10% FCS for 2 days. Cells were pretreated with various concentrations of U0126, SB203580 (SB), SP600125 (SP) or LY2904002 (LY) for 30 min, and stimulated with 100 ng/ml LPS for 20 min (A) or 3 hr (B). A. The cell lysates were subjected to western blot analysis using anti-pERK1/2, ERK1/2, pp38, p38, pJNK1/2, and JNK1/2. B. The gene expression of IL-1β and IL-6 was detected by real time RT-PCR. The mRNA levels were normalized by Ubc mRNA levels. *<i>p</i><0.05 versus control.