PTH treatment enhanced neuroblast migration from the SVZ to the ischemic cortex.

<p>The neuroblast migration and regenerative activity along the ipsilateral SVZ to ischemic cortex migration route was examined by immunostaining of migrating neural progenitor marker DCX (green) and the proliferating marker BrdU (red) 6 days after the onset of ischemia. <b>A</b>. In the post ischemic brain there was significant neuroblast migration from the SVZ to the ischemic barrel cortex (*). Noticeably more DCX and BudU positive cells can be seen in the migration path of PTH treated animals (B) than in the stroke/saline brain (A). <b>C</b>. The total number of DCX+/BrdU+ cells in six sections per animal was counted under a fluorescent microscope. Double-labeling of DCX/BrdU in white matter of ischemic stroke-saline (C) and PTH treated mice (D) 6 days after ischemia. PTH treatment promoted more DCX/BrdU-positive cells moving toward the ischemic cortex (F). The number of DCX+/BrdU+ cells in stroke control and stroke/PTH groups. PTH treatment markedly augmented the proliferating activity among DCX+ cells. <b>D</b>. PTH treatment increased the average migration distance from the SVZ toward the ischemic barrel cortex. N = 6 in stroke/saline group and 7 in stroke/PTH group; * <i>P</i><0.05 vs. stroke controls, ** <i>P</i><0.01 vs. stroke controls.