Effect of lithium (Li) on proliferation of nestin(+) cells following neuronal loss.

<p>Animals were given either lithium carbonate (100 mg/kg, i.p.) or PBS alone with BrdU on day 2 post-treatment with TMT, and then decapitated on day 3 post-treatment for preparation of sagittal hippocampal sections, which were then stained with antibodies against nestin and BrdU (Schedule 1). (<b>a</b>) Fluorescence micrographs show nestin(+) cells (<i>green</i>) and BrdU(+) cells (<i>red</i>) in the dentate gyrus of the 2 groups (impaired/PBS, impaired/Li). Scale bar = 100 µm (<b>b</b>) Graph denoting the number of nestin(+)-BrdU(+) cells in the GCL+SGZ of each group. Values are expressed as the mean ± S.E., calculated from 5 animals.