Expression analyses of chordoma specific and candidate genes in MUG-Chor1 cells.

<p>RT-qPCR was done on AB7900 TaqMan (Applied Biosystems; Foster City, CA). <i>GAPDH</i> and <i>ACTB</i> were used for normalization. Normalization and statistical analysis was done with GenEx Professional (MultiD Analysis; Version 5.3.5.6). All non-template controls were undetermined (Cq>45) except for <i>GAPDH</i> showing two replicates with Cq values >37 and <i>VIM</i> yielding one replicate at Cq = 27. Cut-off for multiple testing (<i>ALG11</i>, <i>UCHL3</i>, <i>TMEM144</i> and <i>PPP2CB</i>) was p = 0.01274.</p>a<p>calculated as mean values from quadruplicate or triplicate (in case the Cq value could not be defined) biological samples.</p>b<p>Cq values were normalized to <i>GAPDH</i> and <i>ACTB</i> (ΔCq). Differential expression (ΔΔCq) is given as positive (up-regulated in large cells) or negative (down-regulated in large cells) fold change ( = 2^ΔΔCq).</p>c<p>Cq values w/o outlier. Outliers were identified by means of Grubbs’ outlier test.